Automatic Fed-Batch Cultivation Enhances Microbial Lipid Production from Volatile Fatty Acids

Organic waste is generated worldwide, and its disposal recycling are becoming a challenge. Due to high carbon content, however, it may be converted into valuable products. Carbon neutrality essential, unstable international oil prices stress the increasing importance of biofuels significantly. Volatile fatty acids (VFA) derived from organic can microbial lipids by oleaginous yeast using as source. When VFA consumed yeast, pH medium rises; hence, acidic agents have added maintain broth’s pH. In this study, we enhanced lipid productivity automatic fed-batch cultivation an agent, modified showed 48.9% 69.0% higher biomass than manual multi-fed culture. At concentration 5 g/L 7.0, yield 0.25 g/g alongside 0.11 g/L/h was obtained system. Oleic acid accounted for largest proportion lipids, composition suitable biodiesel production.